1st International Halal conference , 2017-12-13

Title : ( Dietary inclusion of bull testicles meal on growth performance and immune function of rainbow trout, Oncorhynchus mykiss (Walbaum) )

Authors: Omid Safari , Reza Valizadeh , M. Barkhordar , Hamidreza Ahmadniaye Motlagh ,

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Introduction: The domesticated animal (sheep and bull) production industry produces a large volume of testicles as processing by-products annually. Testicle is considered haram (not kosher) in Islam to eat. There has been an increasing interest to utilize these by-products in aquafeeds as a fish meal substitution or feed additive. Prebiotics, probiotics, parabiotics, synbiotics, organic salts, phyto-products and nucleotides are regarded as the most important feed additives in aquaculture industry. Testes meal from pink salmon (Oncorhynchus gorbuscha) has a nutrient profile similar to fish meal with high levels of crude protein, a well balance amino acid profile and up to ten times the purine content of commercial fish meals. The non-specific immunostimulatory benefits of exogenous dietary nucleotides in fish after feeding rainbow trout with a diet containing supplemental nucleotides was reported. Inclusion of testes meal in a fish meal based diet significantly increased both feed intake and final weight in rainbow trout. Also, dietary supplementation of exogenous nucleotides reduced stress parameters and increased weight gain after vaccination and salt water transfer of Atlantic salmon (Salmo salar). A variety of innate and adaptive immune parameters show changes when nucleotides are added to the diet including phagocytic activity, serum lysozyme and antibody levels. Therefore, based on the high nucleotide content of testes meal and possible immune benefits of milt proteins this study was conducted to examine the potential of bull testes meal as a dietary immunostimulant and functional feedstuff. Methods: Bull testicle (OTe) was oven-dried at 24°C for 71 h. OTe was replaced with fish meal at levels of 10, 20, 30, 40, 50, 70 and 100% based on the protein content. Growth performance, immune responses and disease resistance of juvenile rainbow trout (35.8±0.8 g) fed the test diets for 126 days were compared to control diet. Also, apparent digestibility coefficient (ADC) of crude protein (ADCCP) in rainbow trout fed the test diets was measured. Finally, the test animals were challenged with Aeromonas hydrophila as the causative agent of cold water disease. Results: Using OTe increased (p<0.05) the specific growth rate (SGR), feed conversion ratio (FCR), and immune responses including lysozyme, total immunoglobulin and alternative complement activities compared to those of fed the control diet. Substituting 70% fish meal with OTe showed the highest (p<0.05) final weight, SGR, FCR and all immunity responses. The highest (p<0.05) ADCCP values were measured in rainbow trout fed the 40-100% OTe. The 48-h challenge test showed that feeding rainbow trout with OTE (from 20-100%) increased the resistance rate and survival rate (60-90%) compared to control. Conclusion: The addition of OTe meal to a trout diet had a stimulating effect on the growth performance, immunity and disease resistance of fish the test diets when compared to the control. Therefore, OTe meal can be considered a functional feed ingredient and potential immunostimulant in trout. However, further research is necessary to identify the functional components of OTe and investigate prolonged feeding a diet containing testes meal.


, Rainbow trout, Bull testicle, Growth performance, Stress resistance, Immunity
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author = {Safari, Omid and Valizadeh, Reza and M. Barkhordar and Ahmadniaye Motlagh, Hamidreza},
title = {Dietary inclusion of bull testicles meal on growth performance and immune function of rainbow trout, Oncorhynchus mykiss (Walbaum)},
booktitle = {1st International Halal conference},
year = {2017},
location = {Mashhad, IRAN},
keywords = {Rainbow trout; Bull testicle; Growth performance; Stress resistance; Immunity},


%0 Conference Proceedings
%T Dietary inclusion of bull testicles meal on growth performance and immune function of rainbow trout, Oncorhynchus mykiss (Walbaum)
%A Safari, Omid
%A Valizadeh, Reza
%A M. Barkhordar
%A Ahmadniaye Motlagh, Hamidreza
%J 1st International Halal conference
%D 2017