Journal of Biomedical Materials Research Part A, ( ISI ), Volume (107), No (2), Year (2019-2) , Pages (301-311)

Title : ( Application of mesenchymal stem cells to enhance non-union bone fracture healing )

Authors: Mohamad Mousaei , Maryam Moghaddam Matin , Hossein Kazemi Mehrjerdi , Hojjat Naderi-Meshkin , Ali Moradi , Masoud Rajabioun , Faezeh Alipour , samaneh ghasemi , Mohammad Zare , Mahdi Mirahmadi , Hamid Reza Bidkhori , Ahmad Reza Bahrami ,

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ECM components include a number of osteoinductive and osteoconductive factors, which are involved in bone fracture healing. In this study, a combination of adipose derived mesenchymal stem cells (Ad-MSCs), cancellous bone graft (CBG), and chitosan hydrogel (CHI) was applied to the non-union bone fracture and healing effects were evaluated for the first time. After creation of animal models with nonunion fracture in rats, they were randomly classified into seven groups. Radiography at 0, 2, 4, and 8 weeks after surgery, indicated the positive effects of Ad-MSCs + CBG + CHI and AdMSCs + CBG in treatment of bone fractures as early as 2 weeks after the surgery. These data were confirmed with both biomechanical and histological studies. Gene expression analyses of Vegf and Bmp2 showed a positive effect of Ad-MSCs on vascularization and osteogenic differentiation in all groups receiving Ad-MSCs, as shown by real-time PCR. Immunofluorescence analysis and RT-PCR results indicated existence of human Ad-MSCs in the fractured region 8 weeks post-surgery. In conclusion, we suggest that application of Ad-MSCs, CBG, and CHI, could be a suitable combination for osteoinduction and osteoconduction to improve non-union bone fracture healing. Further investigations are required to determine the exact mechanisms involved in this process before moving to clinical studies. © 2018 Wiley Periodicals, Inc. J. Biomed. Mater. Res. Part A: 107A: 301–311, 2019.


, mesenchymal stem cells, cancellous bone graft, chitosan injectable hydrogel, non-union bone fracture
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author = {Mousaei, Mohamad and Moghaddam Matin, Maryam and Kazemi Mehrjerdi, Hossein and and and Rajabioun, Masoud and Alipour, Faezeh and Ghasemi, Samaneh and and and and Bahrami, Ahmad Reza},
title = {Application of mesenchymal stem cells to enhance non-union bone fracture healing},
journal = {Journal of Biomedical Materials Research Part A},
year = {2019},
volume = {107},
number = {2},
month = {February},
issn = {1549-3296},
pages = {301--311},
numpages = {10},
keywords = {mesenchymal stem cells; cancellous bone graft; chitosan injectable hydrogel; non-union bone fracture},


%0 Journal Article
%T Application of mesenchymal stem cells to enhance non-union bone fracture healing
%A Mousaei, Mohamad
%A Moghaddam Matin, Maryam
%A Kazemi Mehrjerdi, Hossein
%A Rajabioun, Masoud
%A Alipour, Faezeh
%A Ghasemi, Samaneh
%A Bahrami, Ahmad Reza
%J Journal of Biomedical Materials Research Part A
%@ 1549-3296
%D 2019