Trigonella foenum-graecum L. (Fenugreek) is a plant from the family Fabaceae that grows anywhere around the world(1). Fenugreek is commonly used as a traditional food and medicine and is a rich source of steroidal sapogenins, mainly diosgenin that has anti-inflammatory activity and is used for the treatment of leukemia, hypercholesterolemia,climacteric syndrome and colon cancer [2]. 3 different Iranian cultivares of fenugreek including Neyshabour, Alborz and Ardestan were used for this expriment. Concentration of Diosgenin was measured in leaves, stems, roots and seeds in 30, 50 and 70 day plants. The levels of this secondary metabolite were measured by UV-spectrophotometer .The result demonstrated that the concentration of this secondary metabolite varies in different organs and at different stages of growth. The highest content of diosgenin was produced by leaves at 50-day neyshabour cultivare plants.

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