Among hazardous pollutants, 2,4-Dinitrophenol (2,4-DNP) is considered highly toxic and possesses a remarkable resistance to degradation. Therefore, investigation of the possible mechanisms for removal of such pollutants is important. Laccase enzyme can decompose phenolics despite the fact that its application has been limited due to lack of possibility to reuse it. Immobilization can overcome this problem. In this paper, laccase complexes with montmorillonite K10 and zeolite were used to decompose 2,4-DNP with concentrations of 1.5 mg l???? 1 and 50 mg kg???? 1 in synthetic wastewater and soil, respectively. The maximum removal of pollutant from wastewater in samples containing laccase-zeolite and laccase-montmorillonite complexes were 99 and 93.3%, respectively, which occurred at 4 h incubation compared with 6 h for free laccase. The maximum removal of pollutant from soil was observed for all treatments after 16 h of incubation. The maximum removal for samples containing free laccase, laccase-zeolite, and laccase-montmorillonite complexes were 98.5%, 98.6%, and 90.4%, respectively. Control sample also showed maximum removal of 35.8%. In general, application of laccase-zeolite complexes in aqueous environment, and these complexes and free laccases in soil was found very effective in degradation of 2,4-DNP. Hence, the use of laccase, especially immobilized laccases, for removal of 2,4-DNP from environment is promising.

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