Title : ( Multi-walled carbon nanotubes stimulate growth, redox reactions and biosynthesis of antioxidant metabolites in Thymus daenensis celak. in vitro )
Authors: Saba Samadi , Mohammad Jamal Saharkhiz , Majid Azizi , Leila Samiei , Mansour Ghorbanpour ,
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Abstract
This research was aimed at determining the effects of multi-walled carbon nanotubes (MWCNTs) on seed germination, seedling growth parameters and secondary metabolite (SM) production of Thymus daenensis in vitro. Seeds were aseptically cultured in MS medium with various concentrations of MWCNTs (0, 125, 250, 500, 1000 and 2000 μg ml-1). Seed germination and morphological changes in seedlings were measured and the total phenolic contents (TPC) and flavonoids (TFC), antioxidant activities and the activity of polyphenol oxidase (PPO), L-phenylalanine ammonia-lyase (PAL), dehydrogenase (DHA) and peroxidase (POD) enzyme of seedling extract were quantified. Seedling biomass and height significantly improved as the MWCNTs level increased. It peaked at 250 μg ml-1 (0.41 ± 0.01 gr FW, 5.99 ± 0.55 cm) and then rapidly decreased to 0.040 ± 0.1 gr FW and 1.42 ± 0.24 cm in 1000 μg ml-1, 30 days after treatment. Additionally, SM and the analyses of enzyme activity revealed that the highest TPC (6.70 ± 0.06 mg GAE g-1 DW), TFC (8.19 ± 0.01 mg QUE g-1 DW), Antioxidant activities (73.88 ± 0.47 %) and maximum PAL activity (1.25 ± 0.08 mM cm g-1 FW) were detected on MS media fortified with 250 μg ml-1 MWCNTs. The results reveal that MWCNTs in low doses (250 μg ml-1) can encourage the production of biomass, elicit more SM from seedlings and enhance the biosynthesis of antioxidants. TEM images showed that MWCNTs could cross the plant cell wall and enter the cellular cytoplasm.
Keywords
, Elicitor, Multi walled carbon nanotubes, Antioxidants, Enzymes, Secondary metabolite 22 production, Thymus daenensis,
@article{paperid:1078834,
author = {Saba Samadi and Mohammad Jamal Saharkhiz and Azizi, Majid and Samiei, Leila and Mansour Ghorbanpour},
title = {Multi-walled carbon nanotubes stimulate growth, redox reactions and biosynthesis of antioxidant metabolites in Thymus daenensis celak. in vitro},
journal = {Chemosphere},
year = {2020},
volume = {249},
month = {February},
issn = {0045-6535},
keywords = {Elicitor; Multi walled carbon nanotubes; Antioxidants; Enzymes; Secondary metabolite
22 production; Thymus daenensis;},
}
%0 Journal Article
%T Multi-walled carbon nanotubes stimulate growth, redox reactions and biosynthesis of antioxidant metabolites in Thymus daenensis celak. in vitro
%A Saba Samadi
%A Mohammad Jamal Saharkhiz
%A Azizi, Majid
%A Samiei, Leila
%A Mansour Ghorbanpour
%J Chemosphere
%@ 0045-6535
%D 2020
