Although protein electrophoresis has been recently applied as a diagnostic tool in veterinary laboratories, there is no data regarding reference intervals for protein fractions in healthy mynah. To evaluate the electrophoretic pattern of proteins in serum and heparinized-plasma of common mynahs ( Acridotheres tristis ), blood specimens were collected from 55 healthy adult common mynahs (male: 26 and female: 29). The serum total protein and protein fractions were measured using biuret and cellulose acetate electrophoresis (CAE), respectively. Four protein fractions including albumin and alpha (α), beta (β) and gamma (γ) globulins were noticed in the electrophoretogram in serum specimens, and gender had no significant effect on the measured parameters. The serum BCG-Albumin was significantly higher than CAEAlbumin (P = .01). Also, the comparison of total protein and protein fractions in serum and plasma specimens of 25 birds showed that total protein ( d = .66, P = .03), gamma globulin ( d = 1.13, P = .00), and total globulin ( d = .67, P = .00) in plasma samples were significantly higher than those in serum samples. The results of this study provide the specific reference intervals for protein fractions in common mynahs, which are essential for proper interpretation of laboratory results and also revealed that albumin measurement by BCG method yields unreliable results in common mynahs.

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