Biochar (BC) is prepared from waste organic material that can improve soil health in the contaminated area. Soil pollution with cadmium (Cd) is one of the worldwide problems. The present study aimed to evaluate the BC influence on some morphophysiological and biochemical characteristics, also Cd concentration of Ocimum ciliatum L. leaves under Cd stress as well as human risk assessment. Therefore, a pot factorial arrangement based on a completely randomized design was done which included three levels of BC (non-BC, 1, and 2% of the pot soil) and three Cd levels (0, 20, and 40 mg/kg soil) with three replications. The results of the present study indicated that BC application improved morphological traits, photosynthetic pigments, relative water content (RWC), and catalase (CAT) activity of O. ciliatum under Cd stress and reduced total soluble sugars, total phenol, antioxidant activity, proline content, electrolyte leakage (EL), soluble protein content, ascorbate peroxidase (APX), and guaiacol peroxidase (GPX) activities, and Cd concentration as well as target hazard quotient (THQ). In conclusion, based on the findings of this study, BC could be applied as an environmental friendly amendment in Cd-polluted soil to ameliorate the negative influences of Cd stress on O. ciliatum and reduces Cd levels and THQ in the plants due to the absorption properties of BC. This means that BC usage in contaminated soil helps to reduce pollutions and decreases the human risk assessment.

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