Mentha piperita and Shirazi mint are two important cultivars of the Lamiaceae family, which attracted the attention of researchers because of their economic and pharmaceutical importance. Trichoderma is a saprophytic fungus that has a global spread and is seen in almost all agro-soils. This fungus belongs to the Deuteromycota fungus and has about 25 species, the most important of which are T.harizianum, T.viride and T.virenns. Accordingly, the aim of this study was to investigate the effects of various concentrations of Trichoderma fungi Bi isolate on different characteristics of mint (Piperita and Shirazi). The present study was conducted as a pot experiment under non-soil culture conditions in a greenhouse based on a factorial experiment based on a completely randomized design with four concentrations of Trichoderma harizianum Bi: 0% (control), 5%, 10% and 15% vol. 50 liters Water and two cultivars of mint (Piperita and Shirazi) with 4 replications were established and implemented at the research greenhouse of the Faculty of Agriculture, Ferdowsi University of Mashhad,Iran. Based on the results of statistical analysis, the Trichoderma fungus concentration had a significant effect on leaf number and number of nodes. The highest amount of leaf was observed in the concentration of 15% of the fungus, which was observed to be 59.41% higher than the control treatment, where the concentration of fungus was zer (control). In the concentration of fungi, 15% was observed in 143 nodes, which increased 91% relative to control treatment. The concentration of Trichoderma fungus and the type of crop used could have a significant effect on plant height. The highest plant height was 88 cm in the treatment of fungi with a concentration of 15% and Piperita cultivar. The lowest height was observed in Shirazi cultivar and zero concentration of fungus was 22.5 cm. Different concentrations of Trichoderma fungi were not effective on all of the measured biochemical traits. But the type of cultivar used had a significant effect on the amount of chlorophyll a, b. The highest levels of chlorophyll a, b, total in Piperita cultivar were observed, which increased compared to Shirazi cultivar. None of the treatments had significant effect on carotenoid level.

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