Teucrium polium is a medicinal plant endemic to Iran. Due to harsh environmental conditions, it is very difficult to propagate this plant through seed. Therefore, in vitro cultivation is essential for the conservation and propagation of this species. We investigated the effect of different concentrations of BAP and NAA on callogenesis (calli percentage, callus fresh and dry weight) of leaf explants in vitro. First, the leaves as explant was taken from the plant cultivated under hydroponic condition. The samples were sterilized and cultured on MS medium congaing different concentrations of BAP (0. 0.5, 1, and 1.5 mg L-1) and NAA (0, 0.5, and 1 mg L-1). The results showed that different hormonal levels have significant effects on calli percentage, fresh and dry weight of explants. The highest percentage of callus production (100%) was observed in MS containing “BAP” and “BAP+NAA” concentrations, but the greatest positive effect on the fresh and dry weight of calluses was in the combination of BAP1.5 + NAA0.5 (mg L-1). It is worth noting that the lowest callogenesis traits was observed in the “NAA treatments”. Therefore, MS medium with the combination of BAP and NAA is recommended for successful callus formation from the leaf explant of T. polium.

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