In the present study, the phytochemical features of the essential oils from leaves, flowers, and fruits in three Ferulago species including F. angulata, F. carduchorum, and F. contracta was investigated. The essential oils were extracted using the hydrodistillation‏ ‏method and analyzed by GC-FID and GC/MS. The essential oil content varied between 0.46 to 2.65% (v/w) among the various parts of the studied species. Monoterpene hydrocarbons (leaves: 34.80-78.76%, flowers: 88.67-94.26%, fruits: 89.54-94.21%) constituted the main fraction of three Ferulago species. The major compounds of the essential oil among the species were α-pinene (15.01-22.16%), β-phellandrene (2.48-14.73%), α-phellandrene (0.52-13.8%), and germacrene B (0.11-13.28%) in leaves; (Z)-β-ocimene (38.46-47.21%), α-pinene (10.25-18.32%), and α-phellandrene (5.07-9.44%) in flowers; (Z)-β-ocimene (10.21-41.19%), α-phellandrene (7.51-31.89%), α-pinene (8.96-17.71%), β-phellandrene (7.24-17.44%), terpinolene (2.90-7.77%), and δ-3-carene (1.57-7.66%) in fruits. The classical antioxidant activity of the essential oils was assessed using the DPPH method. The fruit essential oils of the studied species induced a significant level of antioxidant activity compared to ascorbic acid (EC50 = 50.24 μg/mL). The highest level of antioxidant activity was found in F. carduchorum (EC50 = 68.75 μg/mL) fruits essential oil. Ferulago species, therefore, have the potential to be utilized as natural antioxidants in the pharmaceutical, food or cosmetic industries owing to their high level of antioxidant activity.

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