Chitosan-based films are promising active biodegradable materials with the ability to be enhanced by different materials, including gums. This study aims to optimize the physical (transmittance, water vapor permeability and water solubility), mechanical (tensile strength and elongation at break) and antibacterial (against Staphylococcus aureus and Salmonella Typhimurium) properties of newly fabricated chitosan/olibanum gum (CH/OG) films as a function of different levels of CH (0.5, 0.75, 1, 1.25 and 1.5% w/v) and OG (0.125, 0.25, 0.375, 0.5 and 0.625% w/v), using response surface methodology (RSM). Moreover, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and differential scanning calorimetry (DSC) were used to better characterize the fabricated films. RSM analysis results showed the significant fitting of all dependent variable responses to the quadratic polynomial model. To attain the desirable physical, mechanical and antibacterial responses, the optimal concentrations were 1.31% (w/v) CH and 0.3% (w/v) OG. The encouraging antibacterial, physical and mechanical properties of the developed composites support the application of chitosan/gum blends in active food packaging, particularly for perishable foodstuffs, such as meat and horticultural products.

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