Trichoderma species are known to have a high potential for biotechnological applications. β-gluco- sidase enzyme is an important component of cellulase enzyme complex of Trichoderma with a crucial role in enzymatic degradation which is inhibited by glucose. The purpose of this study was to find the Trichoderma strains with high cellulase activity and tolerance to glucose. Therefore, 77 Trichoderma isolates were obtained from forest soils in Iran. Ten of them, had high cellulase activity, and the H1N44 isolate showed the highest activity of cellulase as well as high tolerance to glucose. In addition using FPAse assay, the level of cellulase activity was 0.66 U/mL in H1N44 and this result was 4-fold higher than β-glucosidase activity in Trichoderma reesei QM6a in the absence of glucose. Then, 10 representative cellulolytic isolates were sequenced in three genomic loci, including ITS, tef-1 and rpb-2 and H1N44 isolate was recognized as Trichoderma koningiopsis. As a result of this study, H1N44 was identified as a novel cellulolytic and glucose-tolerant strain with high potential for biotechnological utilization

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