LWT- Food Science and Technology, ( ISI ), Volume (191), Year (2024-1)

Title : ( Investigating the effect of Lactiplantibacillus plantarum TW57-4 in preventing biofilm formation and expression of virulence genes in Listeria monocytogenes ATCC 19115 )

Authors: Arezou Rouhi , Fereshteh Falah , marjan azghandi , Berooz Alizadeh Behbahani , Sayed Ali Mortazavi , Farideh Tabatabaei yazdi , Alireza Vasiee ,

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Abstract

Listeria monocytogenes is a common foodborne pathogen associated with listeriosis, a severe illness with a mortality rate of 20–30%. This study aimed to assess the antimicrobial properties of Lactiplantibacillus plantarum TW57-4 cell-free supernatant (CFS) against L. monocytogenes ATCC 19115. The CFS exhibited a minimum inhibitory concentration (MIC) of 31.24 mg/mL against L. monocytogenes. Scanning electron microscopy confirmed its anti-biofilm effects. The impact of CFS on various aspects of L. monocytogenes, such as biofilm formation, auto-aggregation, cytotoxicity, hemolysin activity, adhesion, and invasion, was also investigated. CFS treatment effectively inhibited biofilm formation at concentrations ranging from 1/16MIC (9.67%) to MIC (80%). It also reduced preexisting biofilms by 4.2%–70.91%. The CFS demonstrated cytotoxic effects on Caco-2 cancer cells (IC50, 44.64 mg/mL) and diminished hemolytic activity of L. monocytogenes at concentrations from MIC to 1/4 MIC, with reduction percentages of 50.66% and 28.66% respectively. Moreover, CFS treatment suppressed the expression of virulence genes, inhibiting the pathogenicity of L. monocytogenes. In conclusion, Lpb. plantarum TW57-4 exhibits favorable probiotic properties, antibacterial effects, and holds potential as a natural bacteriostatic agent. Further research and practical applications are warranted. It also shows promise as a bio-intervention for controlling L. monocytogenes biofilm in the food industry.

Keywords

, Listeria monocytogenes CFS Biofilm Pathogenicity RT, qPCR
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@article{paperid:1097123,
author = {Rouhi, Arezou and Falah, Fereshteh and Azghandi, Marjan and بهروز علیزاده بهبانی and Mortazavi, Sayed Ali and Tabatabaei Yazdi, Farideh and علیرضا وسیعی},
title = {Investigating the effect of Lactiplantibacillus plantarum TW57-4 in preventing biofilm formation and expression of virulence genes in Listeria monocytogenes ATCC 19115},
journal = {LWT- Food Science and Technology},
year = {2024},
volume = {191},
month = {January},
issn = {0023-6438},
keywords = {Listeria monocytogenes CFS Biofilm Pathogenicity RT-qPCR},
}

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%0 Journal Article
%T Investigating the effect of Lactiplantibacillus plantarum TW57-4 in preventing biofilm formation and expression of virulence genes in Listeria monocytogenes ATCC 19115
%A Rouhi, Arezou
%A Falah, Fereshteh
%A Azghandi, Marjan
%A بهروز علیزاده بهبانی
%A Mortazavi, Sayed Ali
%A Tabatabaei Yazdi, Farideh
%A علیرضا وسیعی
%J LWT- Food Science and Technology
%@ 0023-6438
%D 2024

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