Aim: The present study was conducted to produce a new carrier containing whey protein isolate-basil seed gum (WPI-BSG) conjugate to achieve superior physicochemical stability of emulsions containing vitamin D3 (Vit-D3). Methods: Zeta-potential and particle size analysis, spectrophotometric method, encapsulation efficiency, loading capacity and dialysis bag method were used to examined physicochemical stability and Vit-D3 release from the emulsions. Results: The conjugate-stabilised emulsion showed maximum encapsulation efficiency (87.05±3.37% (w/w)) and loading capacity (5.43±0.08% (w/w)) at the Vit-D3 concentration of 200 and 300mg/kg. This emulsion also demonstrated good physical stability after 30days of storage with the zeta potential and mean droplet size of −79.60±0.62mV and 1346.82±5.95nm, respectively. Additionally, the conjugate-stabilised emulsion had a maximum Vit-D3 retention (chemical stability) of 72.79±3.58% after a 15-day storage period. Conclusion: Our findings suggest that the conjugate-stabilised emulsion has a good stabilising capacity as a carrier for hydrophobic compounds such as Vit-D3.

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