Title : Frequency of bovine Iymphocyte antigen DRB3.2 alleles in Sarabi cows ( Frequency of bovine Iymphocyte antigen DRB3.2 alleles in Sarabi cows )
Authors: Jalil Tavakkoli Afshari , Mahdi KHOSRAVI , Mohammadreza Nassiri , Feradoun EFTEKHARI SHAHROUDI , Balal Sadeghi , Reza Tohidi ,
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Abstract
The second exon of the bovine Major Histocompati- bility Complex (MHC) class II DRB3 gene was amplified by polymerase chain reaction (PCR) from 50 DNA samples of Iranian Sarabi cattle. Bovine DNA was isolated from aliquots of whole blood. A two-step polymerase chain reaction followed by digestion with restriction endonucleases RsaI, BstyI, and HaeIII was conducted on DNA from samples. Fifteen Bovine Lymphocyte Antigen (BoLA)-DRB3 alleles were assigned, including some that were only recently described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.23. The most frequent alleles were *52 (frequency = 0.23), *11 (0.18) and *23 (0.15). Results of this study demonstrate that the BoLA-DRB3.2 locus is highly polymorphic in Sarabi cattles.Keywords: BoLA-DRB3.2, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), Iranian Sarabi cows.Keywords: BoLA-DRB3.2, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), Iranian Sarabi cows
Keywords
, bovine lymphocyte antigen, PCR- RFLP, Iranian Holstein cattle, polymorphism.
@article{paperid:201928,
author = {Jalil Tavakkoli Afshari and Mahdi KHOSRAVI and Nassiri, Mohammadreza and EFTEKHARI SHAHROUDI, Feradoun and Sadeghi, Balal and Reza Tohidi},
title = {Frequency of bovine Iymphocyte antigen DRB3.2 alleles in Sarabi cows},
journal = {Iranian Journal of Biotechnology},
year = {2004},
volume = {2},
number = {2},
month = {March},
issn = {1728-3043},
pages = {101--105},
numpages = {4},
keywords = {bovine lymphocyte antigen; PCR- RFLP; Iranian Holstein cattle; polymorphism.},
}
%0 Journal Article
%T Frequency of bovine Iymphocyte antigen DRB3.2 alleles in Sarabi cows
%A Jalil Tavakkoli Afshari
%A Mahdi KHOSRAVI
%A Nassiri, Mohammadreza
%A EFTEKHARI SHAHROUDI, Feradoun
%A Sadeghi, Balal
%A Reza Tohidi
%J Iranian Journal of Biotechnology
%@ 1728-3043
%D 2004
