Title : ( In vitro rumen fungi quantification in medium containing sodium hydroxide or formaldehyde treated sunflower meal using quantitative competitive PCR assay )
Authors: Mohsen Danesh Mesgaran , , Alireza Heravi Moussavi , Mohammadreza Nassiri , , Adham Fani Maleki , morteza chaji , AbdulRaoof Alshawkany ,
Full TextAbstract
The rumen anaerobic fungi are primary colonizers of fibrous plant materials that degrade lignin-containing plant cell walls. With the advancement of molecular enumeration methods, in particular 18S rDNA gene probing methods, researchers were able to monitor fungal species within the rumen (Stahl et al., 1998). Quantitative competitive PCR (QCPCR) techniques play an important role in nucleic acid quantification because of their significant lower cost of equipment and consumables (Franz et al., 2001). The aim of this study was to use the QC-PCR assay to evaluate the relative quantitative comparison of anaerobic fungi population in medium containing sunflower meal, either untreated or treated with formaldehyde (3 and 6 g/kg DM) or sodium hydroxide (40 g/kg DM).
Keywords
, rumen fungi , sodium hydroxide, formaldehyde, sunflower meal, competitive PCR
@inproceedings{paperid:1010797,
author = {Danesh Mesgaran, Mohsen and , and Heravi Moussavi, Alireza and Nassiri, Mohammadreza and , and Fani Maleki, Adham and Chaji, Morteza and Alshawkany, AbdulRaoof},
title = {In vitro rumen fungi quantification in medium containing sodium hydroxide or formaldehyde treated sunflower meal using quantitative competitive PCR assay},
booktitle = {British Society of Animal Science 2009},
year = {2009},
location = {ENGLAND},
keywords = {rumen fungi ; sodium hydroxide; formaldehyde; sunflower meal; competitive PCR},
}
%0 Conference Proceedings
%T In vitro rumen fungi quantification in medium containing sodium hydroxide or formaldehyde treated sunflower meal using quantitative competitive PCR assay
%A Danesh Mesgaran, Mohsen
%A ,
%A Heravi Moussavi, Alireza
%A Nassiri, Mohammadreza
%A ,
%A Fani Maleki, Adham
%A Chaji, Morteza
%A Alshawkany, AbdulRaoof
%J British Society of Animal Science 2009
%D 2009
