Title : ( PCR-restriction endonuclease analysis for strain typing of Mycobacterium avium subsp. paratuberculosis based on polymorphisms in IS 1311 )
Authors: Mahdi Soltani , Mohammadreza Nassiri , Balal Sadeghi , Shahrokh Ghovvati Roudsari ,
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Abstract
Point mutations in the IS 1311 sequences from sheep and cattle strains of Mycobacterium avium subsp. paratuberculosis (Map) were targeted to develop a polymerase chain reaction (PCR) that would be useful in the diagnosis and control of Johne’s disease. PCR/REA strategy based on amplifying a 268 bp fragment of IS1311 and digestion by HinfI was developed. Results showed that all of positive results were assigned to cattel strain.
Keywords
, Mycobacterium, PCR-restriction, polymorphisms in IS 1311
@inproceedings{paperid:1016500,
author = {Soltani, Mahdi and Nassiri, Mohammadreza and Sadeghi, Balal and Ghovvati Roudsari, Shahrokh},
title = {PCR-restriction endonuclease analysis for strain typing of Mycobacterium avium subsp. paratuberculosis based on polymorphisms in IS 1311},
booktitle = {European Association Animal Science-EAAP 2008},
year = {2008},
keywords = {Mycobacterium;PCR-restriction;polymorphisms in IS 1311},
}
%0 Conference Proceedings
%T PCR-restriction endonuclease analysis for strain typing of Mycobacterium avium subsp. paratuberculosis based on polymorphisms in IS 1311
%A Soltani, Mahdi
%A Nassiri, Mohammadreza
%A Sadeghi, Balal
%A Ghovvati Roudsari, Shahrokh
%J European Association Animal Science-EAAP 2008
%D 2008
