Title : ( A multiplex polymerase chain reaction for the identification of cows’ and goats’ milk in sheep’s milk )
Authors: Saeid Khanzadi , Jamshid Razmyar , Abdollah Jamshidi , Mohammad Mohsenzadeh ,
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Abstract
Purpose or AIM: The objective of this study was to evaluate the applicability of PCR assay for detection of cow’s and goat’s milk sold as sheep milk. Methods and Materials or CASE: Multiplex PCR assay using species specific primers was used to identify cows’, goats’ and sheep’s somatic cells presented in their milk. In this study 25 milk samples labeled as \"sheep milk\" were purchased from different commercial sources in Mashhad city. After DNA extraction the samples were examined by the multiplex PCR assay using primers targeting the mitochondrial 12s and 16s rRNA gene. RESULTS: The results showed 25% of examined milk samples were proven to be pure sheep milk. Also 25% and 50% of samples were confirmed to be mixed goat and sheep, and cow, goat and sheep milk respectively. DISCUSSION: In conclusion, the multiplex PCR assays used in this study can be useful for milk inspection to detect cow’s and goat’s milk in sheep milk.
Keywords
, Multiplex PCR, Adulteration, Caw Milk, Goat Milk, Sheep Milk
@inproceedings{paperid:1022261,
author = {Khanzadi, Saeid and Razmyar, Jamshid and Jamshidi, Abdollah and Mohsenzadeh, Mohammad},
title = {A multiplex polymerase chain reaction for the identification of cows’ and goats’ milk in sheep’s milk},
booktitle = {European Biotechnology Congress 2011},
year = {2011},
location = {استانبول},
keywords = {Multiplex PCR; Adulteration; Caw Milk; Goat Milk; Sheep Milk},
}
%0 Conference Proceedings
%T A multiplex polymerase chain reaction for the identification of cows’ and goats’ milk in sheep’s milk
%A Khanzadi, Saeid
%A Razmyar, Jamshid
%A Jamshidi, Abdollah
%A Mohsenzadeh, Mohammad
%J European Biotechnology Congress 2011
%D 2011
