Purpose or AIM: The objective of this study was to evaluate the applicability of PCR assay for detection of cow’s and goat’s milk sold as sheep milk. Methods and Materials or CASE: Multiplex PCR assay using species specific primers was used to identify cows’, goats’ and sheep’s somatic cells presented in their milk. In this study 25 milk samples labeled as \"sheep milk\" were purchased from different commercial sources in Mashhad city. After DNA extraction the samples were examined by the multiplex PCR assay using primers targeting the mitochondrial 12s and 16s rRNA gene. RESULTS: The results showed 25% of examined milk samples were proven to be pure sheep milk. Also 25% and 50% of samples were confirmed to be mixed goat and sheep, and cow, goat and sheep milk respectively. DISCUSSION: In conclusion, the multiplex PCR assays used in this study can be useful for milk inspection to detect cow’s and goat’s milk in sheep milk.

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