ABSTRACT: In order to improve in vitro culture of Sebri pear cultivar several combinations of plant growth regulators of BAP, IBA and GA3 were tested. In all PGRs combination, regenerated shoots increased with increasing BAP concentration In media containing of different combinations of BAP (3 mgL-1) and BAP (2 mgL-1) + GA3 the highest of regenerated shoots were obtained. The higher regenerated shoot length showed in media containing IBA. The highest regenerated shoots length obtained in BAP (2 mgL-1) + IBA and the lowest showed in BAP (3 mgL-1). In BAP (3 mgL-1) + GA3 combination, internode length was the highest. Hyperhydricity rate was affected by BAP concentration and plant growth regulators combinations. A negative relation was obtained in internode length and hyperhydricity rate. The higher concentration of BAP induced more hyperhydricity rate and BAP + IBA + GA3 produced the highest hyperhydric regenerated shoots, while BAP (2 mgL-1) + GA3 induced the lowest hyperhydricity rate.

Keywords