An experiment was conducted to investigate the effect of acid-binding capacity (ABC) and acidifiers in different basal diets on leg bones of broilers at 21 and 41 days of age. In a 3 × 2 × 2 factorial experiment, three levels of ABC (650, 800, and 950 mEq/kg), two mixes of acidifiers (fumaric acid and sodium diacetate and tartaric acid and calcium propionate), and two types of basal diet (optimal diet based on corn and suboptimal diet based on wheat–corn without enzyme) were assessed. Wheat–corn diet containing 650 mEq/kg ABC and tartaric acid caused the most density of femur, but fumaric acid resulted in the lowest density as compared with the other treatments at 21 days. The combination of fumaric acid and calcium carbonate improved bone density and ash percentage of tibia at 21 days. Tibia of chickens fed corn diet containing 650 mEq/kg ABC and fumaric acid had the highest density but with tartaric acid had the lowest density as compared with the other dietary treatments. However, the main effects and their interactions were not pronounced on weight, volume, and length of tibia at 21 days. Wheat–corn diet and 950 mEq/kg ABC significantly decreased phosphorus content of serum. However, the other factors and their interactions did not affect the phosphorus and calcium content of serum at 41 days. In histological traits of tibia, corn diet as compared with wheat–corn diet increased the height of proliferative zone but did not affect the hypertrophic and mineralized zones at 21 days. Tartaric acid as compared with fumaric acid significantly increased hypertrophic and mineralized zones but did not affect the proliferative zone at 21 days. The ABC at the level of 950 mEq/kg ABC significantly increased the height of hypertrophic zone but decreased proliferative and mineralized zones at 21 days. These factors and their interactions did not affect any zones of growth plate of tibia at 41 days.

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