Iranian Journal of Veterinary Medicine, Volume (8), No (3), Year (2014-11) , Pages (213-218)

Title : ( Intestinal colonization of different Brachyspira spp. in laying hens )

Authors: , Abdollah Jamshidi , Saeid Khanzadi , Jamshid Razmyar ,

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Abstract

BACKGROUND:Avian intestinal spirochetosis (AIS) is caused by spiral-shaped Gram-negative Brachyspira spp. in poultry and is known as a cause of diarrhea, low egg production, and increased occurrence of dirty eggs in layer hens. OBJECTIVES: In this study, the presence of some Brachyspira spp. was investigated in laying hens. METHODS: A total of 100 cloacal swab samples were individually collected from 20 laying hen flocks showing fecal egg staining in northeastern Iran. RESULTS: Using culture and morphologic examination, 41 samples (41%) from 20 flocks were positive; however, by using genus-specific PCR, only 37 (37%) samples were confirmed as Brachyspira spp. Using speciesspecific primers, single colonization was identified in 18 samples associated with B. pilosicoli (48.6%), while single colonization with B. intermedia was found in only two samples (5.4%). Simultaneous colonization by B. intermedia and B. murdochii was detected in 3 samples (8.1%). B. pilosicoli was the most prevalent species in concurrent colonization in 11 cases (29.7%). Finally, cocolonization by B. intermedia and B. innocens was identified in 3 samples (8.1%). CONCLUSIONS: The results of this study showed the colonization of different species of Brachyspira with dominance of B. pilosicoli in layer hens.

Keywords

, Brachyspira, intestinal spirochetosis, laying hen, PCR
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@article{paperid:1044101,
author = {, and Jamshidi, Abdollah and Khanzadi, Saeid and Razmyar, Jamshid},
title = {Intestinal colonization of different Brachyspira spp. in laying hens},
journal = {Iranian Journal of Veterinary Medicine},
year = {2014},
volume = {8},
number = {3},
month = {November},
issn = {2251-8894},
pages = {213--218},
numpages = {5},
keywords = {Brachyspira; intestinal spirochetosis;laying hen; PCR},
}

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%0 Journal Article
%T Intestinal colonization of different Brachyspira spp. in laying hens
%A ,
%A Jamshidi, Abdollah
%A Khanzadi, Saeid
%A Razmyar, Jamshid
%J Iranian Journal of Veterinary Medicine
%@ 2251-8894
%D 2014

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