Title : ( Differential expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells cultured with hydatid cyst derived antigens )
Authors: Alireza Haghparast , nooshinmehr soleymani , Hassan Borji , Abolghasem Naghibi , fatemeh taran , محمد حسین ناظم شیرازی , مریم ترابی ,
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Abstract
Recognition of helminth derived pathogen associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), including toll like receptors (TLRs) is the first step towards initiating anti-helminth immune responses. Understanding the molecular basis of innate immune recognition of different stages of parasite antigens will provide a better understanding of disease pathogenicity as well as the potential immunostimulatory and/or immunoregulatory roles of these antigens. Using hydatid cyst derived antigens of Echinococcus granulosus, causative agent of cystic echinococcosis, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analysed by real-time quatitative reverse-transcription polymerase
Keywords
, chain reaction (qRT-PCR). Somatic and exceretory/secretory antigens of protoscoleces (PSC), germainal layer (GL) and hydatid cyst fluid (HCF) antigens were prepared to stimulate ovine PBMCs. A higher expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of PSC, whereas onl
@inproceedings{paperid:1046081,
author = {Haghparast, Alireza and Soleymani, Nooshinmehr and Borji, Hassan and Naghibi, Abolghasem and Taran, Fatemeh and محمد حسین ناظم شیرازی and مریم ترابی},
title = {Differential expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells cultured with hydatid cyst derived antigens},
booktitle = {15th International Congress of Immunology},
year = {2013},
location = {میلان, ITALY},
keywords = {chain reaction (qRT-PCR). Somatic and exceretory/secretory antigens of protoscoleces (PSC); germainal layer (GL) and hydatid cyst fluid (HCF) antigens were prepared to stimulate ovine PBMCs. A higher expression of TLR2 and TLR4 was observed in PBMCs cultured with somatic antigens of PSC; whereas only TLR2 was up-regulated by ES antigens of PSC. Similarly; we detected an increased level of TLR2 and TLR4 expression in PBMCs cultured with GL antigens. In contrast; the expression of TLR2 and TLR4 in PBMCs was down-regulated by HCF antigens. The results presented in this study suggest that; PSC (Somatic and excretory/secretory antigen) and GL antigens have immunostimulatory effects whereas HCF antigens exhibit an immunoregulatory effect on peripheral immune cells. In conclusion; this study provide for the first time evidence of induction of TLRs in ovine PBMCs by PSC; GL and HCF antigens of Echinococcus.
P4.07.15},
}
%0 Conference Proceedings
%T Differential expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells cultured with hydatid cyst derived antigens
%A Haghparast, Alireza
%A Soleymani, Nooshinmehr
%A Borji, Hassan
%A Naghibi, Abolghasem
%A Taran, Fatemeh
%A محمد حسین ناظم شیرازی
%A مریم ترابی
%J 15th International Congress of Immunology
%D 2013
