Archives of Razi Institute, ( ISI ), Volume (71), No (1), Year (2016-1) , Pages (15-19)

Title : ( Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine )

Authors: Reza Pasandideh , Mohammadreza Nassiri , maseod raoof delgosha , Ali Asghar Aslaminejad , Mojtaba Tahmoorespur , S. Zibaei , Mohammad Doosti , M. Pasandideh ,

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Abstract

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed livestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs with FMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after the first vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN- γ, TNF-α and IL-10 expression using relative real-time PCR assay. Our results showed that all of the genes were upregulated. The expression of TNF-α and IL-10 genes significantly increased (P<0.05) in day 28th in comparison to the day 7th post the first vaccination. It can be concluded that the vaccine induced immune responses by increasing expression of the cytokines. Therefore, effects of DNA vaccines on immune system also may be evaluated using these genes.

Keywords

, Cytokine, mRNA expression, FMD, Guinea pig, Real-time PCR
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@article{paperid:1055552,
author = {Pasandideh, Reza and Nassiri, Mohammadreza and Raoof Delgosha, Maseod and Aslaminejad, Ali Asghar and Tahmoorespur, Mojtaba and S. Zibaei and Doosti, Mohammad and M. Pasandideh},
title = {Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine},
journal = {Archives of Razi Institute},
year = {2016},
volume = {71},
number = {1},
month = {January},
issn = {0365-3439},
pages = {15--19},
numpages = {4},
keywords = {Cytokine; mRNA expression; FMD; Guinea pig; Real-time PCR},
}

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%0 Journal Article
%T Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine
%A Pasandideh, Reza
%A Nassiri, Mohammadreza
%A Raoof Delgosha, Maseod
%A Aslaminejad, Ali Asghar
%A Tahmoorespur, Mojtaba
%A S. Zibaei
%A Doosti, Mohammad
%A M. Pasandideh
%J Archives of Razi Institute
%@ 0365-3439
%D 2016

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