Neuropeptide Y (NPY) plays a mediatory role on cerebral insulin function in maintaining energy balance. The current study was deigned to determine the role of insulin in food intake and its interaction with NPY receptors in 8 experiments of broiler cockerels,(each with 4 treatment groups except 8). Chickens received control solution, 2.5, 5, and 10 ng of insulin in experiment 1 and control solution, 1.25, 2.5 and 5 μg of B5063, SF22 and SML0891 in experiments 2, 3 and 4 through intracerebroventricular (ICV) injection, respectively. In experiments 5, 6 and 7 chickens received ICV injection of B5063, SF22, SML0891, co-injection of them +insulin, control solution and insulin. In experiment 8, blood glocuse was measured. Insulin, B5063 and SML0891 decreased food intake, while SF22 led to increase in food intake. Also, hypophagic effect of insulin was reinforced with injection of B560, but ICV injection of SF22 destroyed hypophagic effect of insulin and increased food intake (p<0.05). However, SML0891 had no effect on decreased food intake induced by insulin(p>0.05). 30 minutes after injection, blood sugar in control group was higher than insulin group (p<0.05). Therefore, the NPY1 and NPY2 receptors act as mediators in hypophagic effect of insulin in broiler cockerels.

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