Title : ( Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens )
Authors: abas tanhaeian , marjan azghandi , Jamshid Razmyar , el mo , Mohammad Hadi Sekhavati ,
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Abstract
Over the last decades, poultry industry faced to the rapid emergence of multidrug-resistant bacteria as a global concern. Antimicrobial peptide (AMPs) known as potential antibiotic alternative and were considered as a new antimicrobial agent. Current methods of production and purification of AMPs have several limitations such as: costly, time-consuming and killing the producing host cells in recombinant form. In the present study, a chimeric peptide derived from camel lactoferrin was produced in Escherichia coli periplasmic space using a pET-based expression system and its antibacterial activity was determined on some avian pathogens in vitro. A carboxyterminal polyhistidine tag was used for purification by Ni2+ affinity chromatography with an average yield of 0.42 g/L. The His-tagged chimeric peptide showed different range of antimicrobial activity against clinically isolated avian pathogens with low chicken blood hemolysis activity and high serum stability. Overall, the results of this investigation showed the recombinant chimeric peptide was successfully expressed in pET-based expression system and could be considered as a proper alternative for some currently used antibiotics in poultry industry and drugs veterinary medicine.
Keywords
Camel lactoferrin Antimicrobial peptide Periplasmic expression Avian pathology
@article{paperid:1071394,
author = {Tanhaeian, Abas and Azghandi, Marjan and Razmyar, Jamshid and Mo, El and Sekhavati, Mohammad Hadi},
title = {Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens},
journal = {Micobial Pathogenesis},
year = {2018},
volume = {122},
number = {5},
month = {September},
issn = {0882-4010},
pages = {73--78},
numpages = {5},
keywords = {Camel lactoferrin
Antimicrobial peptide
Periplasmic expression
Avian pathology},
}
%0 Journal Article
%T Recombinant production of a chimeric antimicrobial peptide in E. coli and assessment of its activity against some avian clinically isolated pathogens
%A Tanhaeian, Abas
%A Azghandi, Marjan
%A Razmyar, Jamshid
%A Mo, El
%A Sekhavati, Mohammad Hadi
%J Micobial Pathogenesis
%@ 0882-4010
%D 2018
