tBacteria of the Pseudomonas putida group are studied for a large panel of properties ranging from plantgrowth promotion and bioremediation to pathogenicity. To date, most of the classification of individ-ual pseudomonads from this group relies on 16S RNA gene analysis, which is insufficient for accuratetaxonomic characterization within bacterial species complexes of the Pseudomonas putida group. Here,a collection of 20 of these bacteria, isolated from various soils, was assessed via multi-locus sequenceanalysis of rpoD, gyrB and rrs genes. The 20 strains clustered in 7 different clades of the P. putida group.One strain per cluster was sequenced and results were compared to complete genome sequences of typestrains of the P. putida group. Phylogenetic analyses, average nucleotide identity data and digital DNAhybridizations, combined to phenotypic characteristics, resulted in the proposition and description offour new species i.e. Pseudomonas alloputida Kh7T(= LMG 29756T= CFBP 8484T) sp. nov., Pseudomonasinefficax JV551A3T(= DSM108619T= CFBP 8493T) sp. nov., Pseudomonas persica RUB6T(= LMG 29757T= CFBP 8486T) sp. nov. and Pseudomonas shirazica VM14T(= LMG 29953T= CFBP 8487T) sp. nov.

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