Introduction: Biological scaffolds materials derived from the extracellular matrix (ECM) have been created the most promising evolution in the field of tissue engineering. it seems that a clear understanding of such a matrix and attempt to make them applicable will let us to discover important advances in tissue engineering and regenerative medicine. This study was aimed to prepare a cellular matrix from bovine articular cartilage, bone epiphysial, media layer of bovine aortic and gingival tissue. Methods: In order to remove cells from the desired tissue, the physico-chemical methods was used, which were including: Snap freezing and using sodium Dodecyle sulfate (SDS). In addition, elastic scaffold have been prepared from aorta by using solution of 50 mg/ml 70% bromide Syanogen and formic acid. Thus, porous scaffold with a thickness of about 2 to 3 mm were prepared. To study the quality of these scaffolds and comparison the cell behaviors into 3dimensional matrix, desired scaffolds were cultured with blastema tissue which was prepared as a ring by punching the pinnas of New Zealand white rabbits. Interaction between acellular cancellous bovin bone and blastema tissue was evaluated by histochemical techniques such as Picrofushin, hematoxylin and eosin (H&E), picrosirius red, orcein and also DAPI flurescent staining in different days (10, 20, 30 and 40). Conclusion: It has been shown that this scaffold could be a suitable model in evaluating the cellular behaviors such as cell division, migration, adhesion and differentiation. it seems that these 3extracellular matrix can serve as a suitable model for advanced study in stem cell biology.

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