Title : ( Implication of complex vertebral malformation and deficiency of uridine monophosphate synthase on molecular-based testing in the Iranian Holstein bulls population )
Authors: , Mohammadreza Nassiri , Balal Sadeghi , , Mojtaba Tahmoorespur , Reza Valizadeh ,
Full TextAbstract
The aim of this study was to identify the deficiency of uridine monophosphate synthase (DUMPS) and the complex vertebral malformation (CVM) in Iranian Holstein bulls. A total of 144 blood samples were prepared of Holstein bulls in Abbas Abad Animal Breeding Center and Ferdowsi University of Mashhad\'s Dairy Farm in Khorasan state of Iran. Genomic PCR-RFLP protocol was performed to amplify the polymorphic region of the bovine uridine monophosphate synthase UMPS gene. Also, genomic PCR-SSCP method was performed for CVM to amplify the polymorphic region of the bovine solute carrier family 35 member 3 (SLC35A3) genes. The results of this study demonstrated that there was no carrier of DUMPS and CVM in Iranian bulls in these centers.
Keywords
, DUMPS, CVM, RFLP, SSCP, Iranian Holstein bulls
@article{paperid:1012834,
author = {, and Nassiri, Mohammadreza and Sadeghi, Balal and , and Tahmoorespur, Mojtaba and Valizadeh, Reza},
title = {Implication of complex vertebral malformation and deficiency of uridine monophosphate synthase on molecular-based testing in the Iranian Holstein bulls population},
journal = {African Journal of Biotechnology},
year = {2009},
volume = {8},
number = {22},
month = {November},
issn = {1684-5315},
pages = {6077--6081},
numpages = {4},
keywords = {DUMPS; CVM; RFLP; SSCP; Iranian Holstein bulls},
}
%0 Journal Article
%T Implication of complex vertebral malformation and deficiency of uridine monophosphate synthase on molecular-based testing in the Iranian Holstein bulls population
%A ,
%A Nassiri, Mohammadreza
%A Sadeghi, Balal
%A ,
%A Tahmoorespur, Mojtaba
%A Valizadeh, Reza
%J African Journal of Biotechnology
%@ 1684-5315
%D 2009
